Ontology highlight
ABSTRACT:
SUBMITTER: Burmann F
PROVIDER: S-EPMC5344682 | biostudies-literature | 2017 Mar
REPOSITORIES: biostudies-literature
Bürmann Frank F Basfeld Alrun A Vazquez Nunez Roberto R Diebold-Durand Marie-Laure ML Wilhelm Larissa L Gruber Stephan S
Molecular cell 20170223 5
SMC proteins support vital cellular processes in all domains of life by organizing chromosomal DNA. They are composed of ATPase "head" and "hinge" dimerization domains and a connecting coiled-coil "arm." Binding to a kleisin subunit creates a closed tripartite ring, whose ∼47-nm-long SMC arms act as barrier for DNA entrapment. Here, we uncover another, more active function of the bacterial Smc arm. Using high-throughput genetic engineering, we resized the arm in the range of 6-60 nm and found th ...[more]