Urinary microbiome of kidney transplant patients reveals dysbiosis with potential for antibiotic resistance.

ABSTRACT: Recent studies have established that a complex community of microbes colonize the human urinary tract; however, their role in kidney transplant patients treated with prophylactic antibiotics remains poorly investigated. Our aim was to investigate the urinary microbiome of kidney transplant recipients. Urine samples from 21 patients after kidney transplantation and 8 healthy controls were collected. All patients received prophylactic treatment with the antibiotic combination trimethoprim-sulfamethoxazole. Metagenomic DNA was isolated from urine samples, sequenced using shotgun sequencing approach on Illumina HiSeq 2000 platform, and analyzed for microbial taxonomic and functional annotations. Our results demonstrate that the urine microbiome of kidney transplants was markedly different at a

SUBMITTER: Rani A 

PROVIDER: S-EPMC5344767 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature