Stabilizing short-lived Schiff base derivatives of 5-aminouracils that activate mucosal-associated invariant T cells.
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ABSTRACT:
SUBMITTER: Mak JY
PROVIDER: S-EPMC5344979 | biostudies-literature | 2017 Mar
REPOSITORIES: biostudies-literature
Publications
Stabilizing short-lived Schiff base derivatives of 5-aminouracils that activate mucosal-associated invariant T cells.
Mak Jeffrey Y W JY Xu Weijun W Reid Robert C RC Corbett Alexandra J AJ Meehan Bronwyn S BS Wang Huimeng H Chen Zhenjun Z Rossjohn Jamie J McCluskey James J Liu Ligong L Fairlie David P DP
Nature communications 20170308
Mucosal-associated invariant T (MAIT) cells are activated by unstable antigens formed by reactions of 5-amino-6-D-ribitylaminouracil (a vitamin B2 biosynthetic intermediate) with glycolysis metabolites such as methylglyoxal. Here we show superior preparations of antigens in dimethylsulfoxide, avoiding their rapid decomposition in water (t<sub>1/2</sub> 1.5 h, 37 °C). Antigen solution structures, MAIT cell activation potencies (EC<sub>50</sub> 3-500 pM), and chemical stabilities are described. Co ...[more]
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Project description: Not available
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