Long-range compaction and flexibility of interphase chromatin in budding yeast analyzed by high-resolution imaging techniques.

ABSTRACT: Little is known about how chromatin folds in its native state. Using optimized in situ hybridization and live imaging techniques have determined compaction ratios and fiber flexibility for interphase chromatin in budding yeast. Unlike previous studies, ours examines nonrepetitive chromatin at intervals short enough to be meaningful for yeast chromosomes and functional domains in higher eukaryotes. We reconcile high-resolution fluorescence in situ hybridization data from intervals of 14-100 kb along single chromatids with measurements of whole chromosome arms (122-623 kb in length), monitored in intact cells through the targeted binding of bacterial repressors fused to GFP derivatives. The results are interpreted with a flexible polymer model and suggest that interphase chromatin exists in

SUBMITTER: Bystricky K 

PROVIDER: S-EPMC534505 | biostudies-literature | 2004 Nov

REPOSITORIES: biostudies-literature