Lah is a transmembrane protein and requires Spa10 for stable positioning of Woronin bodies at the septal pore of Aspergillus fumigatus.

ABSTRACT: Woronin bodies are specialized, fungal-specific organelles that enable an immediate closure of septal pores after injury to protect hyphae from excessive cytoplasmic bleeding. In most Ascomycetes, Woronin bodies are tethered at the septal pore by so-called Lah proteins. Using the pathogenic mold Aspergillus fumigatus as a model organism, we show that the C-terminal 288 amino acids of Lah (LahC₂₈₈) bind to the rim of the septal pore. LahC₂₈₈ essentially consists of a membrane spanning region and a putative extracellular domain, which are both required for the targeting to the septum. In an A. fumigatus rho4 deletion mutant that has a severe defect in septum formation, LahC₂₈₈ is recruited to spot-like structures in or at the lateral membrane. This suggests t

SUBMITTER: Leonhardt Y 

PROVIDER: S-EPMC5345055 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature