Kinetic ¹⁵N-isotope effects on algal growth.

ABSTRACT: Stable isotope labeling is a standard technique for tracing material transfer in molecular, ecological and biogeochemical studies. The main assumption in this approach is that the enrichment with a heavy isotope has no effect on the organism metabolism and growth, which is not consistent with current theoretical and empirical knowledge on kinetic isotope effects. Here, we demonstrate profound changes in growth dynamics of the green alga Raphidocelis subcapitata grown in ¹⁵N-enriched media. With increasing ¹⁵N concentration (0.37 to 50 at%), the lag phase increased, whereas maximal growth rate and total yield decreased; moreover, there was a negative relationship between the growth and the lag phase across the treatments. The latter suggests that a trade-off between gr

SUBMITTER: Andriukonis E 

PROVIDER: S-EPMC5345060 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature