LINE-1 is preferentially hypomethylated within adenomatous polyps in the presence of synchronous colorectal cancer.

ABSTRACT:

Background

Conventional tubular adenomas are frequently detected in patients undergoing average risk screening colonoscopy and are over-represented in patients who will develop colorectal cancer (CRC). Whether features of adenomas could serve as predictors of synchronous CRC is not known. Here, we investigate whether global methylation markers, including LINE-1, differ within adenomas in patients with and without synchronous CRC.

Methods

Colorectal tubular/tubulovillous adenomatous polyps in the absence (P group, n = 45) and in the presence of synchronous CRC (PC group, n = 32) were identified. Global methylation and demethylation by ELISA for 5-methylcytosine (5-mC) and 5-hydroxymethyl cytosine (5-hmC), respectively, were assessed in polyps and adjacent normal

SUBMITTER: Jiang AC 

PROVIDER: S-EPMC5345219 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature