Adipocyte-specific deletion of mTOR inhibits adipose tissue development and causes insulin resistance in mice.

ABSTRACT:

Aims/hypothesis

The in vivo role of mechanistic target of rapamycin (mTOR) in the development and function of adipose tissue, especially brown adipose tissue (BAT), is not well understood. Here, we aimed to assess the effect of mTOR (also known as Mtor) knockout on adipose tissues and systemic energy metabolism.

Methods

We generated adipocyte-specific mTOR-knockout mice (Adipoq-mTOR) by crossing adiponectin-Cre (Adipoq-Cre) mice with mTOR (flox/flox) mice. The mice were then subjected to morphological, physiological (indirect calorimetry, glucose and insulin tolerance tests) and gene expression analyses to determine the role of mTOR in adipose tissues.

Results

We provide in vivo evidence that mTOR is essential for adipose tissue development and growth. Deletion of mTO

SUBMITTER: Shan T 

PROVIDER: S-EPMC5345851 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature