Comparison of Glutamate Turnover in Nerve Terminals and Brain Tissue During [1,6-¹³C₂]Glucose Metabolism in Anesthetized Rats.

ABSTRACT: The ¹³C turnover of neurotransmitter amino acids (glutamate, GABA and aspartate) were determined from extracts of forebrain nerve terminals and brain homogenate, and fronto-parietal cortex from anesthetized rats undergoing timed infusions of [1,6-¹³C₂]glucose or [2-¹³C]acetate. Nerve terminal ¹³C fractional labeling of glutamate and aspartate was lower than those in whole cortical tissue at all times measured (up to 120 min), suggesting either the presence of a constant dilution flux from an unlabeled substrate or an unlabeled (effectively non-communicating on the measurement timescale) glutamate pool in the nerve terminals. Half times of ¹³C labeling from [1,6-¹³C₂]glucose, as estimated by least square

SUBMITTER: Patel AB 

PROVIDER: S-EPMC5345906 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature