PowerPlex® Fusion 6C System: evaluation study for analysis of casework and database samples.

ABSTRACT:

Aim

To report on the successful analysis of amplicons obtained with PowerPlex® Fusion 6C System, a highly robust 27-plex genotyping kit developed for human identification laboratories, on the Applied Biosystems® 3500 Genetic Analyzer.

Method

We performed characterization and evaluation studies following the Scientific Working Group on DNA Analysis Methods (SWGDAM) validation guidelines, examining several critical areas of kit performance. We report the results of sensitivity, robustness, heterozygous peak height ratio, precision, concordance, caseworks, and mixture interpretations. We tested sensitivity, using serial dilutions of control DNA.

Results

The minimum amount of input DNA resulting in a full profile was 125 pg. Inhibition, inducted by urea, showsed a progres

SUBMITTER: Cisana S 

PROVIDER: S-EPMC5346900 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature