PET Cell Tracking Using ¹⁸F-FLT is Not Limited by Local Reuptake of Free Radiotracer.

ABSTRACT: Assessing the retention of cell therapies following implantation is vital and often achieved by labelling cells with 2'-[¹⁸F]-fluoro-2'-deoxy-D-glucose (¹⁸F-FDG). However, this approach is limited by local retention of cell-effluxed radiotracer. Here, in a preclinical model of critical limb ischemia, we assessed a novel method of cell tracking using 3'-deoxy-3'-L-[¹⁸F]-fluorothymidine (¹⁸F-FLT); a clinically available radiotracer which we hypothesise will result in minimal local radiotracer reuptake and allow a more accurate estimation of cell retention. Human endothelial cells (HUVECs) were incubated with ¹⁸F-FDG or ¹⁸F-FLT and cell characteristics were evaluated. Dynamic positron emission tomography (PET) images were acqui

SUBMITTER: MacAskill MG 

PROVIDER: S-EPMC5347009 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature