High-throughput screening of rare metabolically active tumor cells in pleural effusion and peripheral blood of lung cancer patients.

ABSTRACT: Malignant pleural effusion (MPE), the presence of malignant cells in pleural fluid, is often the first sign of many cancers and occurs in patients with metastatic malignancies. Accurate detection of tumor cells in pleural fluid is crucial because the presence of MPE denotes an advanced stage of disease and directs a switch in clinical managements. Cytology, as a traditional diagnostic tool, has limited sensitivity especially when tumor cells are not abundant, and may be confounded by reactive mesothelial cells in the pleural fluid. We describe a highly sensitive approach for rapid detection of metabolically active tumor cells in MPE via exploiting the altered glucose metabolism of tumor cells relative to benign cells. Metabolically active tumor cells with high glucose uptake, as evaluated

SUBMITTER: Tang Y 

PROVIDER: S-EPMC5347549 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature