Single-neuron identification of chemical constituents, physiological changes, and metabolism using mass spectrometry.

ABSTRACT: The use of single-cell assays has emerged as a cutting-edge technique during the past decade. Although single-cell mass spectrometry (MS) has recently achieved remarkable results, deep biological insights have not yet been obtained, probably because of various technical issues, including the unavoidable use of matrices, the inability to maintain cell viability, low throughput because of sample pretreatment, and the lack of recordings of cell physiological activities from the same cell. In this study, we describe a patch clamp/MS-based platform that enables the sensitive, rapid, and in situ chemical profiling of single living neurons. This approach integrates modified patch clamp technique and modified MS measurements to directly collect and detect nanoliter-scale samples from the cytoplasm

SUBMITTER: Zhu H 

PROVIDER: S-EPMC5347563 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature