Integrated view of internal friction in unfolded proteins from single-molecule FRET, contact quenching, theory, and simulations.

ABSTRACT: Internal friction is an important contribution to protein dynamics at all stages along the folding reaction. Even in unfolded and intrinsically disordered proteins, internal friction has a large influence, as demonstrated with several experimental techniques and in simulations. However, these methods probe different facets of internal friction and have been applied to disparate molecular systems, raising questions regarding the compatibility of the results. To obtain an integrated view, we apply here the combination of two complementary experimental techniques, simulations, and theory to the same system: unfolded protein L. We use single-molecule Förster resonance energy transfer (FRET) to measure the global reconfiguration dynamics of the chain, and photoinduced electron transfer (PET), a

SUBMITTER: Soranno A 

PROVIDER: S-EPMC5347633 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature