Proteomic characterization of microdissected breast tissue environment provides a protein-level overview of malignant transformation.

ABSTRACT: Both healthy and cancerous breast tissue is heterogeneous, which is a bottleneck for proteomics-based biomarker analysis, as it obscures the cellular origin of a measured protein. We therefore aimed at obtaining a protein-level interpretation of malignant transformation through global proteome analysis of a variety of laser capture microdissected cells originating from benign and malignant breast tissues. We compared proteomic differences between these tissues, both from cells of epithelial origin and the stromal environment, and performed string analysis. Differences in protein abundances corresponded with several hallmarks of cancer, including loss of cell adhesion, transformation to a migratory phenotype, and enhanced energy metabolism. Furthermore, despite enriching for (tumor) epithel

SUBMITTER: Braakman RB 

PROVIDER: S-EPMC5347865 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature