Extensive translational regulation during seed germination revealed by polysomal profiling.

ABSTRACT: This work investigates the extent of translational regulation during seed germination. The polysome occupancy of each gene is determined by genome-wide profiling of total mRNA and polysome-associated mRNA. This reveals extensive translational regulation during Arabidopsis thaliana seed germination. The polysome occupancy of thousands of individual mRNAs changes to a large extent during the germination process. Intriguingly, these changes are restricted to two temporal phases (shifts) during germination, seed hydration and germination. Sequence features, such as upstream open reading frame number, transcript length, mRNA stability, secondary structures, and the presence and location of specific motifs correlated with this translational regulation. These features differed significantly betwe

SUBMITTER: Bai B 

PROVIDER: S-EPMC5347915 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature