Overexpression of a C₄-dicarboxylate transporter is the key for rerouting citric acid to C₄-dicarboxylic acid production in Aspergillus carbonarius.

ABSTRACT:

Background

C₄-dicarboxylic acids, including malic acid, fumaric acid and succinic acid, are valuable organic acids that can be produced and secreted by a number of microorganisms. Previous studies on organic acid production by Aspergillus carbonarius, which is capable of producing high amounts of citric acid from varieties carbon sources, have revealed its potential as a fungal cell factory. Earlier attempts to reroute citric acid production into C₄-dicarboxylic acids have been with limited success.

Results

In this study, a glucose oxidase deficient strain of A. carbonarius was used as the parental strain to overexpress a native C₄-dicarboxylate transporter and the gene frd encoding fumarate reductase from Trypanosoma brucei individually and

SUBMITTER: Yang L 

PROVIDER: S-EPMC5348913 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature