Balanced CoQ₆ biosynthesis is required for lifespan and mitophagy in yeast.

ABSTRACT: Coenzyme Q is an essential lipid with redox capacity that is present in all organisms. In yeast its biosynthesis depends on a multiprotein complex in which Coq7 protein has both catalytic and regulatory functions. Coq7 modulates CoQ₆ levels through a phosphorylation cycle, where dephosphorylation of three amino acids (Ser/Thr) by the mitochondrial phosphatase Ptc7 increases the levels of CoQ₆. Here we analyzed the role of Ptc7 and the phosphorylation state of Coq7 in yeast mitochondrial function. The conversion of the three Ser/Thr to alanine led to a permanently active form of Coq7 that caused a 2.5-fold increase of CoQ₆ levels, albeit decreased mitochondrial respiratory chain activity and oxidative stress resistance capacity. This resulted in an increase

SUBMITTER: Gonzalez-Mariscal I 

PROVIDER: S-EPMC5349121 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature