Liver-specific deletion of the Plpp3 gene alters plasma lipid composition and worsens atherosclerosis in apoE^-/- mice.

ABSTRACT: The PLPP3 gene encodes for a ubiquitous enzyme that dephosphorylates several lipid substrates. Genome-wide association studies identified PLPP3 as a gene that plays a role in coronary artery disease susceptibility. The aim of the study was to investigate the effect of Plpp3 deletion on atherosclerosis development in mice. Because the constitutive deletion of Plpp3 in mice is lethal, conditional Plpp3 hepatocyte-specific null mice were generated by crossing floxed Plpp3 mice with animals expressing Cre recombinase under control of the albumin promoter. The mice were crossed onto the athero-prone apoE^-/- background to obtain Plpp3^f/fapoE^-/-Alb-Cre⁺ and Plpp3^f/fapoE^-/-Alb-Cre^- offspring, the latter of which were used

SUBMITTER: Busnelli M 

PROVIDER: S-EPMC5349609 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature