Thyroid transcription factor FOXE1 interacts with ETS factor ELK1 to co-regulate TERT.

ABSTRACT:

Background

Although FOXE1 was initially recognized for its role in thyroid organogenesis, more recently a strong association has been identified between the FOXE1 locus and thyroid cancer. The role of FOXE1 in adult thyroid, and in particular regarding cancer risk, has not been well established. We hypothesised that discovering key FOXE1 transcriptional partners would in turn identify regulatory pathways relevant to its role in oncogenesis.

Results

In a transcription factor-binding array, ELK1 was identified to bind FOXE1. We confirmed this physical association in heterologously transfected cells by IP and mammalian two-hybrid assays. In thyroid tissue, endogenous FOXE1 was shown to bind ELK1, and using ChIP assays these factors bound thyroid-relevant gene promoters TPO and

SUBMITTER: Bullock M 

PROVIDER: S-EPMC5349888 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature