Porous extraction paddle: a solid-phase extraction technique for studying the urine metabolome.

ABSTRACT:

Rationale

A method was needed to accomplish solid-phase extraction of a large urine volume in a convenient way where resources are limited, towards a goal of metabolome and xenobiotic exposome analysis at another, distant location.

Methods

A porous extraction paddle (PEP) was set up, comprising a porous nylon bag containing extraction particles that is flattened and immobilized between two stainless steel meshes. Stirring the PEP after attachment to a shaft of a motor mounted on the lid of the jar containing the urine accomplishes extraction. The bag contained a mixture of nonpolar and partly nonpolar particles to extract a diversity of corresponding compounds.

Results

Elution of a urine-exposed, water-washed PEP with aqueous methanol containing triethylammonium aceta

SUBMITTER: Shao G 

PROVIDER: S-EPMC5350062 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature