Genetic Tracing of Ca_v3.2 T-Type Calcium Channel Expression in the Peripheral Nervous System.

ABSTRACT: Characterizing the distinct functions of the T-type ion channel subunits Ca_v3.1, 3.2 or 3.3 has proven difficult due to their highly conserved amino-acid sequences and the lack of pharmacological blockers specific for each subunit. To precisely determine the expression pattern of the Ca_v3.2 channel in the nervous system we generated two knock-in mouse strains that express EGFP or Cre recombinase under the control of the Ca_v3.2 gene promoter. We show that in the brains of these animals, the Ca_v3.2 channel is predominantly expressed in the dentate gyrus of the hippocampus. In the peripheral nervous system, the activation of the promoter starts at E9.5 in neural crest cells that will give rise to dorsal root ganglia (DRG) neurons, but not sympatheti

SUBMITTER: Bernal Sierra YA 

PROVIDER: S-EPMC5350092 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature