Genetic Diversity of Brucella Reference and Non-reference Phages and Its Impact on Brucella-Typing.

ABSTRACT: Virulent phages have been used for many years to type Brucella isolates, but until recently knowledge about the genetic makeup of these phages remains limited. In this work the host specificity and genomic sequences of the original set (deposited in 1960) of VLA Brucella reference phages Tb, Fi, Wb, Bk2, R/C, and Iz were analyzed and compared with hitherto described brucellaphages. VLA phages turned out to be different from homonymous phages in other laboratories. The host range of the phages was defined by performing plaque assays with a wide selection of Brucella strains. Propagation of the phages on different strains did not alter host specificity. Sequencing of the phages Tb_V, Fi_V, Wb_V, and R/C_V revealed nucleotide variat

SUBMITTER: Hammerl JA 

PROVIDER: S-EPMC5350156 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature