Orai and TRPC channel characterization in FcεRI-mediated calcium signaling and mediator secretion in human mast cells.

ABSTRACT: Inappropriate activation of mast cells via the FcεRI receptor leads to the release of inflammatory mediators and symptoms of allergic disease. Calcium influx is a critical regulator of mast cell signaling and is required for exocytosis of preformed mediators and for synthesis of eicosanoids, cytokines and chemokines. Studies in rodent and human mast cells have identified Orai calcium channels as key contributors to FcεRI-initiated mediator release. However, until now the role of TRPC calcium channels in FcεRI-mediated human mast cell signaling has not been published. Here, we show evidence for the expression of Orai 1,2, and 3 and TRPC1 and 6 in primary human lung mast cells and the LAD2 human mast cell line but, we only find evidence of functional contribution of Orai and not TRPC channel

SUBMITTER: Wajdner HE 

PROVIDER: S-EPMC5350174 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature