Neurons derived from different brain regions are inherently different in vitro: a novel multiregional brain-on-a-chip.

ABSTRACT: Brain in vitro models are critically important to developing our understanding of basic nervous system cellular physiology, potential neurotoxic effects of chemicals, and specific cellular mechanisms of many disease states. In this study, we sought to address key shortcomings of current brain in vitro models: the scarcity of comparative data for cells originating from distinct brain regions and the lack of multiregional brain in vitro models. We demonstrated that rat neurons from different brain regions exhibit unique profiles regarding their cell composition, protein expression, metabolism, and electrical activity in vitro. In vivo, the brain is unique in its structural and functional organization, and the interactions and communication between different brain areas are essential componen

SUBMITTER: Dauth S 

PROVIDER: S-EPMC5350271 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature