Proteomic and Mutant Analysis of the CO₂ Concentrating Mechanism of Hydrothermal Vent Chemolithoautotroph Thiomicrospira crunogena.

ABSTRACT: Many autotrophic microorganisms are likely to adapt to scarcity in dissolved inorganic carbon (DIC; CO₂ + HCO₃^- + CO₃^2-) with CO₂ concentrating mechanisms (CCM) that actively transport DIC across the cell membrane to facilitate carbon fixation. Surprisingly, DIC transport has been well studied among cyanobacteria and microalgae only. The deep-sea vent gammaproteobacterial chemolithoautotroph Thiomicrospira crunogena has a low-DIC inducible CCM, though the mechanism for uptake is unclear, as homologs to cyanobacterial transporters are absent. To identify the components of this CCM, proteomes of T. crunogena cultivated under low- and high-DIC conditions were compared. Fourteen proteins, including those comprising

SUBMITTER: Mangiapia M 

PROVIDER: S-EPMC5350277 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature