Genome-Wide Transcriptional Response to Varying RpoS Levels in Escherichia coli K-12.

ABSTRACT: The alternative sigma factor RpoS is a central regulator of many stress responses in Escherichia coli The level of functional RpoS differs depending on the stress. The effect of these differing concentrations of RpoS on global transcriptional responses remains unclear. We investigated the effect of RpoS concentration on the transcriptome during stationary phase in rich media. We found that 23% of genes in the E. coli genome are regulated by RpoS, and we identified many RpoS-transcribed genes and promoters. We observed three distinct classes of response to RpoS by genes in the regulon: genes whose expression changes linearly with increasing RpoS level, genes whose expression changes dramatically with the production of only a little RpoS ("sensitive" genes), and genes whose exp

SUBMITTER: Wong GT 

PROVIDER: S-EPMC5350281 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature