TOPBP1^Dpb11 plays a conserved role in homologous recombination DNA repair through the coordinated recruitment of 53BP1^Rad9.

ABSTRACT: Genome maintenance and cancer suppression require homologous recombination (HR) DNA repair. In yeast and mammals, the scaffold protein TOPBP1^Dpb11 has been implicated in HR, although its precise function and mechanism of action remain elusive. In this study, we show that yeast Dpb11 plays an antagonistic role in recombination control through regulated protein interactions. Dpb11 mediates opposing roles in DNA end resection by coordinating both the stabilization and exclusion of Rad9 from DNA lesions. The Mec1 kinase promotes the pro-resection function of Dpb11 by mediating its interaction with the Slx4 scaffold. Human TOPBP1^Dpb11 engages in interactions with the anti-resection factor 53BP1 and the pro-resection factor BRCA1, suggesting that TOPBP1 also mediates opposi

SUBMITTER: Liu Y 

PROVIDER: S-EPMC5350513 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature