Revealing large metagenomic regions through long DNA fragment hybridization capture.

ABSTRACT:

Background

High-throughput DNA sequencing technologies have revolutionized genomic analysis, including the de novo assembly of whole genomes from single organisms or metagenomic samples. However, due to the limited capacity of short-read sequence data to assemble complex or low coverage regions, genomes are typically fragmented, leading to draft genomes with numerous underexplored large genomic regions. Revealing these missing sequences is a major goal to resolve concerns in numerous biological studies.

Methods

To overcome these limitations, we developed an innovative target enrichment method for the reconstruction of large unknown genomic regions. Based on a hybridization capture strategy, this approach enables the enrichment of large genomic regions allowing the reconstruc

SUBMITTER: Gasc C 

PROVIDER: S-EPMC5351058 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature