Production of BP178, a derivative of the synthetic antibacterial peptide BP100, in the rice seed endosperm.

ABSTRACT:

Background

BP178 peptide is a synthetic BP100-magainin derivative possessing strong inhibitory activity against plant pathogenic bacteria, offering a great potential for future applications in plant protection and other fields. Here we report the production and recovery of a bioactive BP178 peptide using rice seeds as biofactories.

Results

A synthetic gene encoding the BP178 peptide was prepared and introduced in rice plants. The gene was efficiently expressed in transgenic rice under the control of an endosperm-specific promoter. Among the three endosperm-specific rice promoters (Glutelin B1, Glutelin B4 or Globulin 1), best results were obtained when using the Globulin 1 promoter. The BP178 peptide accumulated in the seed endosperm and was easily recovered from rice seeds

SUBMITTER: Montesinos L 

PROVIDER: S-EPMC5351061 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature