Exaggerated CpH methylation in the autism-affected brain.

ABSTRACT:

Background

The etiology of autism, a complex, heritable, neurodevelopmental disorder, remains largely unexplained. Given the unexplained risk and recent evidence supporting a role for epigenetic mechanisms in the development of autism, we explored the role of CpG and CpH (H = A, C, or T) methylation within the autism-affected cortical brain tissue.

Methods

Reduced representation bisulfite sequencing (RRBS) was completed, and analysis was carried out in 63 post-mortem cortical brain samples (Brodmann area 19) from 29 autism-affected and 34 control individuals. Analyses to identify single sites that were differentially methylated and to identify any global methylation alterations at either CpG or CpH sites throughout the genome were carried out.

Results

We report that w

SUBMITTER: Ellis SE 

PROVIDER: S-EPMC5351204 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature