Identification of Filamin-A and -B as potential biomarkers for prostate cancer.

ABSTRACT:

Aim

A novel strategy for prostate cancer (PrCa) biomarker discovery is described.

Materials & methods

In vitro perturbation biology, proteomics and Bayesian causal analysis identified biomarkers that were validated in in vitro models and clinical specimens.

Results

Filamin-B (FLNB) and Keratin-19 were identified as biomarkers. Filamin-A (FLNA) was found to be causally linked to FLNB. Characterization of the biomarkers in a panel of cells revealed differential mRNA expression and regulation. Moreover, FLNA and FLNB were detected in the conditioned media of cells. Last, in patients without PrCa, FLNA and FLNB blood levels were positively correlated, while in patients with adenocarcinoma the relationship is dysregulated.

Conclusion

These data support

SUBMITTER: Narain NR 

PROVIDER: S-EPMC5351499 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature