Accurate self-correction of errors in long reads using de Bruijn graphs.

ABSTRACT:

Motivation

New long read sequencing technologies, like PacBio SMRT and Oxford NanoPore, can produce sequencing reads up to 50 000 bp long but with an error rate of at least 15%. Reducing the error rate is necessary for subsequent utilization of the reads in, e.g. de novo genome assembly. The error correction problem has been tackled either by aligning the long reads against each other or by a hybrid approach that uses the more accurate short reads produced by second generation sequencing technologies to correct the long reads.

Results

We present an error correction method that uses long reads only. The method consists of two phases: first, we use an iterative alignment-free correction method based on de Bruijn graphs with increasing length of k -mers, and second, the correct

SUBMITTER: Salmela L 

PROVIDER: S-EPMC5351550 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature