Activation of ATR-Chk1 pathway facilitates EBV-mediated transformation of primary tonsillar B-cells.

ABSTRACT: Primary infection of the immunocompromised host with the oncovirus Epstein-Barr virus (EBV) that targets mainly B-cells is associated with an increased risk for EBV-associated tumors. The early events subsequent to primary infection with potential for B-cell transformation are poorly studied. Here, we modeled in vitro the primary infection by using B-cells isolated from tonsils, the portal of entry of EBV, since species specificity of EBV hampers modeling in experimental animals. Increasing evidence indicates that the host DNA damage response (DDR) can influence and be influenced by EBV infection. Thus, we inoculated tonsillar B-cells (TBCs) with EBV-B95.8 and investigated cell proliferation and the DDR during the first 96 hours thereafter. We identified for the first time that EBV infecti

SUBMITTER: Mordasini V 

PROVIDER: S-EPMC5351645 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature