The isolation of morphologically intact and biologically active extracellular vesicles from the secretome of cancer-associated adipose tissue.

ABSTRACT: Breast cancer cells closely interact with different cell types of the surrounding adipose tissue to favor invasive growth and metastasis. Extracellular vesicles (EVs) are nanometer-sized vesicles secreted by different cell types that shuttle proteins and nucleic acids to establish cell-cell communication. To study the role of EVs released by cancer-associated adipose tissue in breast cancer progression and metastasis a standardized EV isolation protocol that obtains pure EVs and maintains their functional characteristics is required. We implemented differential ultracentrifugation as a pre-enrichment step followed by OptiPrep density gradient centrifugation (dUC-ODG) to isolate EVs from the conditioned medium of cancer-associated adipose tissue. A combination of immune-electron microscopy,

SUBMITTER: Jeurissen S 

PROVIDER: S-EPMC5351718 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature