Differentially expressed miRNAs in sepsis-induced acute kidney injury target oxidative stress and mitochondrial dysfunction pathways.

ABSTRACT:

Objective

To identify specific miRNAs involved in sepsis-induced AKI and to explore their targeting pathways.

Methods

The expression profiles of miRNAs in serum from patients with sepsis-induced AKI (n = 6), sepsis-non AKI (n = 6), and healthy volunteers (n = 3) were investigated by microarray assay and validated by quantitative PCR (qPCR). The targets of the differentially expressed miRNAs were predicted by Target Scan, mirbase and Miranda. Then the significant functions and involvement in signaling pathways of gene ontology (GO) and KEGG pathways were analyzed. Furthermore, eight miRNAs were randomly selected out of the differentially expressed miRNAs for further testing by qPCR.

Results

qPCR analysis confirmed that the expressions levels of hsa-miR-23a-3p, hsa-miR-

SUBMITTER: Ge QM 

PROVIDER: S-EPMC5351858 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature