Study of early stage non-small-cell lung cancer using Orbitrap-based global serum metabolomics.

ABSTRACT:

Purpose

The aim of the project was to apply ultra-high-performance liquid chromatography-quadrupole-Orbitrap-high-resolution mass spectrometry for serum metabolite profiling of non-small-cell lung cancer (NSCLC). This Orbitrap-based methodology has been applied for a study of NSCLC potential markers for the first time.

Methods

After extraction using protein precipitation, sera were separated on the ACE Excel 2 C18-PFP (100 × 2.1 mm, 2.0 µm) column using gradient elution and analyzed within the range of 70-1000 m/z. Only patients with early stage disease (stages IA-IIB) were included in the study, providing opportunity to find biomarkers for early lung cancer detection. The resulting metabolite profiles were subjected to univariate and multivariate statistical tests.

Resul

SUBMITTER: Klupczynska A 

PROVIDER: S-EPMC5352735 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature