Preparation of mucosal nanoparticles and polymer-based inactivated vaccine for Newcastle disease and H9N2 AI viruses.

ABSTRACT:

Aim

To develop a mucosal inactivated vaccines for Newcastle disease (ND) and H9N2 viruses to protect against these viruses at sites of infections through mucosal immunity.

Materials and methods

In this study, we prepared two new formulations for mucosal bivalent inactivated vaccine formulations for Newcastle and Avian Influenza (H9N2) based on the use of nanoparticles and polymer adjuvants. The prepared vaccines were delivered via intranasal and spray routes of administration in specific pathogen-free chickens. Cell-mediated and humoral immune response was measured as well as challenge trial was carried out. In addition, ISA71 water in oil was also evaluated.

Results

Our results showed that the use of spray route as vaccination delivery method of polymer and nanoparti

SUBMITTER: Naggar HM 

PROVIDER: S-EPMC5352844 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature