Cross-species hybridisation of human and bovine orthologous genes on high density cDNA microarrays.

ABSTRACT:

Background

Cross-species gene-expression comparison is a powerful tool for the discovery of evolutionarily conserved mechanisms and pathways of expression control. The usefulness of cDNA microarrays in this context is that broad areas of homology are compared and hybridization probes are sufficiently large that small inter-species differences in nucleotide sequence would not affect the analytical results. This comparative genomics approach would allow a common set of genes within a specific developmental, metabolic, or disease-related gene pathway to be evaluated in experimental models of human diseases. The objective of this study was to investigate the feasibility and reproducibility of cross-species analysis employing a human cDNA microarray as probe.

Results

As a proof o

SUBMITTER: Adjaye J 

PROVIDER: S-EPMC535340 | biostudies-literature | 2004 Oct

REPOSITORIES: biostudies-literature