Engineering fungal de novo fatty acid synthesis for short chain fatty acid production.

ABSTRACT: Fatty acids (FAs) are considered strategically important platform compounds that can be accessed by sustainable microbial approaches. Here we report the reprogramming of chain-length control of Saccharomyces cerevisiae fatty acid synthase (FAS). Aiming for short-chain FAs (SCFAs) producing baker's yeast, we perform a highly rational and minimally invasive protein engineering approach that leaves the molecular mechanisms of FASs unchanged. Finally, we identify five mutations that can turn baker's yeast into a SCFA producing system. Without any further pathway engineering, we achieve yields in extracellular concentrations of SCFAs, mainly hexanoic acid (C₆-FA) and octanoic acid (C₈-FA), of 464 mg l^-1 in total. Furthermore, we succeed in the specific productio

SUBMITTER: Gajewski J 

PROVIDER: S-EPMC5353594 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature