Aptamer-based Field-Effect Biosensor for Tenofovir Detection.

ABSTRACT: During medical treatment it is critical to maintain the circulatory concentration of drugs within their therapeutic range. A novel biosensor is presented in this work to address the lack of a reliable point-of-care drug monitoring system in the market. The biosensor incorporates high selectivity and sensitivity by integrating aptamers as the recognition element and field-effect transistors as the signal transducer. The drug tenofovir was used as a model small molecule. The biointerface of the sensor is a binary self-assembled monolayer of specific thiolated aptamer and 6-mercapto-1-hexanol (MCH), whose ratio was optimized by electrochemical impedance spectroscopy measurements to enhance the sensitivity towards the specific target. Surface plasmon resonance, performed under different buffer

SUBMITTER: Aliakbarinodehi N 

PROVIDER: S-EPMC5353720 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature