Quantitative and correlation analysis of the DNA methylation and expression of DAPK in breast cancer.

ABSTRACT:

Background

Death-associated protein kinase 1 (DAPK) is an important tumor suppressor kinase involved in the regulation of multiple cellular activities such as apoptosis and autophagy. DNA methylation of DAPK gene was found in various types of cancers and often correlated with the clinicopathological characteristics. However, the mRNA and protein expression of DAPK in the same sample was rarely measured. Thus, it was unclear if the correlation between DAPK gene methylation and clinicopathological parameters was due to the loss of DAPK expression.

Methods

In this study, the DNA methylation rate, mRNA and protein expression of DAPK was quantitatively detected in 15 pairs of breast cancer patient samples including tumor (T) and adjacent non-tumor (N) tissues.

Results

The

SUBMITTER: Zhu Y 

PROVIDER: S-EPMC5354070 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature