Down-regulation of the placental BCRP/ABCG2 transporter in response to hypoxia signaling.

ABSTRACT:

Introduction

The BCRP/ABCG2 efflux transporter protects the developing fetus by limiting the transplacental transfer of drugs and chemicals and prevents the apoptosis of trophoblasts. The purpose of this study was to determine whether hypoxia-related signaling alters placental BCRP expression and function in vitro and in human pregnancies.

Methods

Human BeWo choriocarcinoma cells were treated with the hypoxia mimetic, cobalt chloride (CoCl₂), or 3% oxygen for 24-48 h. Activation of HIF-1α signaling and regulation of BCRP was assessed using qPCR, ELISA, western blotting and a fluorescent substrate transport assay. In addition, healthy term placentas from high altitude pregnancies with chronic hypoxia were assessed for BCRP expression.

Results

CoCl₂

SUBMITTER: Francois LN 

PROVIDER: S-EPMC5354084 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature