Cellular enrichment through microfluidic fractionation based on cell biomechanical properties.

ABSTRACT: The biomechanical properties of populations of diseased cells are shown to have differences from healthy populations of cells, yet the overlap of these biomechanical properties can limit their use in disease cell enrichment and detection. We report a new microfluidic cell enrichment technology that continuously fractionates cells through differences in biomechanical properties, resulting in highly pure cellular subpopulations. Cell fractionation is achieved in a microfluidic channel with an array of diagonal ridges that are designed to segregate biomechanically distinct cells to different locations in the channel. Due to the imposition of elastic and viscous forces during cellular compression, which are a function of cell biomechanical properties including size and viscoelasticity, larger,

SUBMITTER: Wang G 

PROVIDER: S-EPMC5354170 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature