Dissection of TALE-dependent gene activation reveals that they induce transcription cooperatively and in both orientations.

ABSTRACT: Plant-pathogenic Xanthomonas bacteria inject transcription activator-like effector proteins (TALEs) into host cells to specifically induce transcription of plant genes and enhance susceptibility. Although the DNA-binding mode is well-understood it is still ambiguous how TALEs initiate transcription and whether additional promoter elements are needed to support this. To systematically dissect prerequisites for transcriptional initiation the activity of one TALE was compared on different synthetic Bs4 promoter fragments. In addition, a large collection of artificial TALEs spanning the OsSWEET14 promoter was compared. We show that the presence of a TALE alone is not sufficient to initiate transcription suggesting the requirement of additional supporting promoter elements. At the OsSWEET14 pro

SUBMITTER: Streubel J 

PROVIDER: S-EPMC5354296 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature