Integrated analysis of promoter mutation, methylation and expression of AKT1 gene in Chinese breast cancer patients.

ABSTRACT:

Background

As downstream mediators of PI3K /PTEN /AKT /mTORC1 pathway, the AKT isoforms play critical roles in tumorgenesis. Although the pleiotropic effects of AKT1 in breast cancer have been reported, the genetic and epigenetic characteristics of AKT1 promoter region in breast cancer remains to be identified. In this study we aimed to investigate the promoter mutation spectrum, methylation and gene expression pattern of AKT1 and their relationship with breast cancer.

Methods

By using PCR target sequence enrichment and next-generation sequencing technology, we sequenced AKT1 promoter region in pairs of breast tumor and normal tissues from 95 unselected Chinese breast cancer patients. The methylation of the promoter region and the expression profile of AKT1 in the same cohor

SUBMITTER: Heng J 

PROVIDER: S-EPMC5354459 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature