Comparative Characterization of Phosphatidic Acid Sensors and Their Localization during Frustrated Phagocytosis.

ABSTRACT: Phosphatidic acid (PA) is the simplest phospholipid naturally existing in living organisms, but it constitutes only a minor fraction of total cell lipids. PA has attracted considerable attention because it is a phospholipid precursor, a lipid second messenger, and a modulator of membrane shape, and it has thus been proposed to play key cellular functions. The dynamics of PA in cells and in subcellular compartments, however, remains an open question. The recent generation of fluorescent probes for PA, by fusing GFP to PA-binding domains, has provided direct evidence for PA dynamics in different intracellular compartments. Here, three PA sensors were characterized in vitro, and their preferences for different PA species in particular lipidic environments were compared. In addition, th

SUBMITTER: Kassas N 

PROVIDER: S-EPMC5354483 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature