Cox1 mutation abrogates need for Cox23 in cytochrome c oxidase biogenesis.

ABSTRACT: Cox23 is a known conserved assembly factor for cytochrome c oxidase, although its role in cytochrome c oxidase (CcO) biogenesis remains unresolved. To gain additional insights into its role, we isolated spontaneous suppressors of the respiratory growth defect in cox23∆ yeast cells. We recovered independent colonies that propagated on glycerol/lactate medium for cox23∆ cells at 37°C. We mapped these mutations to the mitochondrial genome and specifically to COX1 yielding an I¹⁰¹F substitution. The I¹⁰¹F Cox1 allele is a gain-of-function mutation enabling yeast to respire in the absence of Cox23. CcO subunit steady-state levels were restored with the I¹⁰¹F Cox1 suppressor mutation and oxygen consumption and CcO activity were

SUBMITTER: Dela Cruz R 

PROVIDER: S-EPMC5354592 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature