The miR-196b miRNA inhibits the GATA6 intestinal transcription factor and is upregulated in colon cancer patients.

ABSTRACT:

Objective

To explore the possible misexpression of the microRNA miR-196b in colorectal cancer (CRC) and its role in controlling the expression of GATA6, a putative target gene crucial to intestinal cell homeostasis and tumorigenesis.

Design

The expression of miR-196b was analysed by qRT-PCR in surgical resection samples from a cohort of sporadic colon cancer patients. Manipulations of miR-196b expression were performed to demonstrate its inhibition of GATA6 protein levels.

Results

We found that miR-196b is significantly upregulated in pre-treatment surgical resection samples from a cohort of sporadic colon cancer patients. The upregulation of miR-196b correlates with less severe clinicopathological characteristics, such as early tumor stage and absence of lymph node m

SUBMITTER: Fantini S 

PROVIDER: S-EPMC5354868 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature