Analysis of acquired mutations in transgenes arising in Ba/F3 transformation assays: findings and recommendations.

ABSTRACT: The identification and functional validation of potentially oncogenic mutations in leukemia is an essential step toward a future of personalized targeted therapy. To assess the oncogenic capacity of individual mutations, reliable and scalable in vitro experimental approaches are required. Since 1988, researchers have used the IL-3 dependent Ba/F3 transformation assay to validate the oncogenic potential of mutations to drive factor-independent growth. Here we report a previously unrecognized phenomenon whereby Ba/F3 cells, engineered to express weakly transforming mutations, present with additional acquired mutations in the expressed transgene following factor withdrawal. Using four mutations with known transformative capacity in three cytokine receptors (CSF2RB, CSF3R and IL7R), we demonst

SUBMITTER: Watanabe-Smith K 

PROVIDER: S-EPMC5355038 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature